VHL Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2735257

Produktdetails

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 1

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses VHL Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human VHL (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: VHL (Von Hippel-Lindau Tumor Suppressor, E3 Ubiquitin Protein Ligase (VHL))

Andere Bezeichnung: Vhl (VHL Produkte)

Synonyme: HRCA1 Protein, RCA1 Protein, VHL1 Protein, pVHL Protein, BcDNA:RH61560 Protein, CG13221 Protein, DVhl Protein, Dmel\\CG13221 Protein, Dvhl Protein, VHL Protein, d-VHL Protein, d-vhl Protein, dVHL Protein, dmVHL Protein, vhl Protein, rca1 Protein, vhl1 Protein, hrca1 Protein, zgc:158722 Protein, Vhlh Protein, von Hippel-Lindau tumor suppressor Protein, von Hippel-Lindau Protein, von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase Protein, von Hippel-Lindau disease tumor suppressor Protein, VHL Protein, Vhl Protein, vhl Protein, CpipJ_CPIJ009992 Protein

Hintergrund: Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molekulargewicht: 24 kDa

NCBI Accession: NP_000542

Pathways: Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Ubiquitin Proteasome Pathway