UPF3B Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2735037

Produktdetails

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses UPF3B Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human UPF3B / RENT3B (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: UPF3B (UPF3 Regulator of Nonsense Transcripts Homolog B (UPF3B))

Andere Bezeichnung: Upf3b,rent3b (UPF3B Produkte)

Synonyme: HUPF3B Protein, MRXS14 Protein, RENT3B Protein, UPF3X Protein, 5730594O13Rik Protein, AI317193 Protein, AW541158 Protein, RGD1560264 Protein, UPF3B, regulator of nonsense mediated mRNA decay Protein, UPF3 regulator of nonsense transcripts homolog B (yeast) Protein, UPF3B Protein, Upf3b Protein

Hintergrund: This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

Molekulargewicht: 56 kDa

NCBI Accession: NP_075386