UMPS Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2735007

Produktdetails

Target: UMPS (Uridine Monophosphate Synthetase (UMPS))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses UMPS Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human UMPS protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: UMPS activity is verified in a bioassay:

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Bardeleben, Sharma, Reeve, Bassilian, Frost, Hoang, Shi, Lichtenstein: "Metabolomics identifies pyrimidine starvation as the mechanism of 5-aminoimidazole-4-carboxamide-1-β-riboside-induced apoptosis in multiple myeloma cells." in: Molecular cancer therapeutics, Vol. 12, Issue 7, pp. 1310-21, (2013) (PubMed).

Antigendetails

Target: UMPS (Uridine Monophosphate Synthetase (UMPS))

Andere Bezeichnung: Umps (UMPS Produkte)

Synonyme: zgc:55702 Protein, zgc:55723 Protein, UMPS Protein, OPRT Protein, 1700095D23Rik Protein, AA408257 Protein, AL033308 Protein, BB164745 Protein, Orotidine 5'-phosphate decarboxylase Protein, uridine monophosphate synthetase Protein, uridine monophosphate synthetase L homeolog Protein, uridine 5'-monophosphate synthase Protein, umps-1 Protein, umps Protein, umps.L Protein, UMPS Protein, LOC100556619 Protein, Umps Protein

Hintergrund: This gene encodes a uridine 5&apos-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5&apos-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5&apos-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.

Molekulargewicht: 52 kDa

NCBI Accession: NP_000364

Pathways: Ribonucleoside Biosynthetic Process