Synaptosomal-Associated Protein, 25kDa (SNAP25) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)

Details zu Produkt Nr. ABIN2732308, Anbieter: Anmelden zum Anzeigen
Proteinname
  • ric-4
  • ric4
  • sec9
  • snap-25
  • SNAP-25B
  • SNAP-25a
  • Bdr
  • SNAP-25
  • sp
  • RIC-4
  • RIC4
  • SEC9
  • SNAP
  • bA416N4.2
  • dJ1068F16.2
  • fj55h11
  • snap25
  • wu:fj55h11
  • synaptosome associated protein 25kDa S homeolog
  • synaptosome associated protein 25kDa
  • synaptosomal-associated protein 25
  • synaptosome associated protein 25kDa L homeolog
  • synaptosome associated protein 25
  • synaptosomal-associated protein, 25kDa
  • zgc:101731
  • snap25.S
  • snap25
  • BACOIKO003_07
  • Tsp_10811
  • snap25.L
  • Snap25
  • SNAP25
  • zgc:101731
Proteineigenschaft
Transcript Variant 1
14
2
2
1
1
1
1
1
1
Spezies
Human
19
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
HEK-293 Cells
15
9
2
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses SNAP25 Protein ist gelabelt mit Myc-DYKDDDDK Tag.
Applikation
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Spezifität Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
  • Recombinant human SNAP25 (transcript variant 1) protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Reinheit > 80 % as determined by SDS-PAGE and Coomassie blue staining
Proteinname
Hintergrund Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
Molekulargewicht 23.2 kDa
NCBI Accession NP_003072
Pathways Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
Applikations-hinweise Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Kommentare

The tag is located at the C-terminal.

Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Bilder des Herstellers
Western Blotting (WB) image for Synaptosomal-Associated Protein, 25kDa (SNAP25) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag) (ABIN2732308) Validation with Western Blot
Produkt verwendet in: Brinkmalm, Brinkmalm, Honer, Moreno, Jakobsson, Mallucci, Zetterberg, Blennow, Öhrfelt: "Targeting synaptic pathology with a novel affinity mass spectrometry approach." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 10, pp. 2584-92, 2014 (PubMed).

Brinkmalm, Brinkmalm, Honer, Frölich, Hausner, Minthon, Hansson, Wallin, Zetterberg, Blennow, Öhrfelt: "SNAP-25 is a promising novel cerebrospinal fluid biomarker for synapse degeneration in Alzheimer's disease." in: Molecular neurodegeneration, Vol. 9, pp. 53, 2014 (PubMed).