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PTPN1 Protein (His tag)

PTPN1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Produktnummer ABIN2730106
  • Target Alle PTPN1 Proteine anzeigen
    PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 10
    • 1
    • 1
    • 1
    Human
    Quelle
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses PTPN1 Protein ist gelabelt mit His tag.
    Applikation
    Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
    Spezifität
    Optimal preservation of protein structure, post-translational modifications and functions.
    Produktmerkmale
    • Recombinant human PTPN1 protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    Reinheit
    > 90 % as determined by SDS-PAGE and Coomassie blue staining
    Biological Activity Comment
    Specific activity: ~14000 nmol/min/mg. Enzymatic activity was determined by measuring the amount of 4-Nitrophenol hydrolyzed from p-nitrophenyl phosphate (pNPP) at 30C.
    Top Product
    Discover our top product PTPN1 Protein
  • Applikationshinweise
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Kommentare

    The tag is located at the N-terminal.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    > 50 μg/mL
    Buffer
    25 mM HEPES, 50 mM NaCl, 2.5 mM EDTA, PH7.5,5 mM DTT. < td valign=top>
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    PTPN1 (Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1))
    Andere Bezeichnung
    Ptpn1 (PTPN1 Produkte)
    Synonyme
    PTPN1 Protein, PTP1B Protein, PTP-1B Protein, PTP-HA2 Protein, Ptp Protein, ptp1b Protein, wu:fk54h03 Protein, CPTP1 Protein, protein tyrosine phosphatase, non-receptor type 1 Protein, protein tyrosine phosphatase, non-receptor type 1 L homeolog Protein, PTPN1 Protein, Ptpn1 Protein, ptpn1 Protein, ptpn1.L Protein
    Hintergrund
    The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
    Molekulargewicht
    39.2 KDa
    NCBI Accession
    NP_002818
    Pathways
    TLR Signalweg, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling
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