PICALM Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2728884

Produktdetails

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 1

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PICALM Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human PICALM (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Zhao, Sagare, Ma, Halliday, Kong, Kisler, Winkler, Ramanathan, Kanekiyo, Bu, Owens, Rege, Si, Ahuja, Zhu, Miller, Schneider, Maeda, Maeda, Sugawara, Ichida, Zlokovic: "Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance." in: Nature neuroscience, Vol. 18, Issue 7, pp. 978-87, (2015) (PubMed).

Antigendetails

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Andere Bezeichnung: Picalm (PICALM Produkte)

Synonyme: LAP-3 Protein, PEPS Protein, CALM Protein, CLTH Protein, LAP Protein, PAP180 Protein, fit-1 Protein, fit1 Protein, mKIAA4114 Protein, Calm Protein, leucine aminopeptidase 3 Protein, phosphatidylinositol binding clathrin assembly protein Protein, si:ch211-200p22.4 Protein, LAP3 Protein, PICALM Protein, Picalm Protein, si:ch211-200p22.4 Protein

Hintergrund: This gene encodes a clathrin assembly protein, which recruits clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. The protein may be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. The protein is involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. A chromosomal translocation t(1011)(p13q14) leading to the fusion of this gene and the MLLT10 gene is found in acute lymphoblastic leukemia, acute myeloid leukemia and malignant lymphomas. The polymorphisms of this gene are associated with the risk of Alzheimer disease. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 70.6 kDa

NCBI Accession: NP_009097

Pathways: Synaptic Membrane