PDGFA Protein (Transcript Variant 2)

Produktnummer ABIN2728534

Produktdetails

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human PDGFA (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is less than or equal to 1 ng/ml, corresponding to a specific activity of > 1 x 10^6 units/mg.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handhabung: Resuspend the protein in the desired concentration in proper buffer

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Andere Bezeichnung: Pdgfa (PDGFA Produkte)

Synonyme: PDGF-A Protein, PDGF1 Protein, pdgfa Protein, pdgfa-2 Protein, zgc:100777 Protein, pdgf Protein, pdgf1 Protein, pdgf-a Protein, AA409063 Protein, CD142 Protein, Cf-3 Protein, Cf3 Protein, TF Protein, PDGFACP Protein, wu:fc32c12 Protein, zgc:153249 Protein, platelet derived growth factor subunit A Protein, platelet-derived growth factor alpha polypeptide a Protein, coagulation factor III Protein, platelet derived growth factor, alpha Protein, platelet derived growth factor subunit A S homeolog Protein, platelet-derived growth factor alpha polypeptide b Protein, PDGFA Protein, pdgfaa Protein, pdgfa Protein, F3 Protein, Pdgfa Protein, pdgfa.S Protein, pdgfab Protein

Hintergrund: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 28.5 kDa

NCBI Accession: NP_002598

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling