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HNF4A Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

HNF4A Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD, Func, PI Active
Produktnummer ABIN2722902
  • Target Alle HNF4A Proteine anzeigen
    HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    Transcript Variant 2
    Spezies
    • 8
    • 1
    • 1
    • 1
    Human
    Quelle
    • 4
    • 2
    • 2
    • 2
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses HNF4A Protein ist gelabelt mit Myc-DYKDDDDK Tag.
    Applikation
    Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)
    Spezifität
    Optimal preservation of protein structure, post-translational modifications and functions.
    Produktmerkmale
    • Recombinant human HNF4 alpha / TCF14 (transcript variant 2) protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    Reinheit
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Biological Activity Comment
    HNF4A Activity Verified in a DNA-binding Assay: HNF4A (, transcript variant 2) activity was measured in a colorimetric DNA-binding assay. Purified HNF4A protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the HNF4A consensus DNA-binding sequence (see below). Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence.
  • Applikationshinweise
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Kommentare

    The tag is located at the C-terminal.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    > 50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Ning, Koh, Pan, Jeong: "Hepatocyte nuclear factor (HNF) 4α transactivation of cytochrome P450 (Cyp) 2d40 promoter is enhanced during pregnancy in mice." in: Biochemical pharmacology, Vol. 94, Issue 1, pp. 46-52, (2015) (PubMed).

  • Target
    HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))
    Andere Bezeichnung
    Hnf4 Alpha,tcf14 (HNF4A Produkte)
    Synonyme
    HNF4A Protein, HNF4alpha Protein, CG9310 Protein, DmHNF4 Protein, Dmel\\CG9310 Protein, HNF-4 Protein, HNF-4(D) Protein, HNF4 Protein, Hnf-4 Protein, Hnf-4h Protein, NR2A4 Protein, dHNF-4 Protein, dHNF4 Protein, Hnf4 Protein, Hnf4alpha Protein, MODY1 Protein, Nr2a1 Protein, Tcf14 Protein, HNF4a7 Protein, HNF4a8 Protein, HNF4a9 Protein, MODY Protein, NR2A1 Protein, NR2A21 Protein, TCF Protein, TCF14 Protein, HNF-4alpha Protein, fb58h09 Protein, id:ibd1279 Protein, wu:fb58h09 Protein, hnf4 Protein, nr2a1 Protein, hepatocyte nuclear factor 4 alpha Protein, Hepatocyte nuclear factor 4 Protein, hepatic nuclear factor 4, alpha Protein, hepatocyte nuclear factor 4, alpha Protein, hepatocyte nuclear factor 4 alpha S homeolog Protein, HNF4A Protein, hnf4a Protein, Hnf4 Protein, Hnf4a Protein, hnf4a.S Protein
    Hintergrund
    Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. [UniProtKB/Swiss-Prot Function]
    Molekulargewicht
    52.6 kDa
    NCBI Accession
    NP_000448
    Pathways
    AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process
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