GATA4 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2721732

Produktdetails

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses GATA4 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human GATA4 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: GATA4 Activity Verified in a DNA-binding Assay: Comparison of GATA4 binding to consensus and mutant oligos shows sensitivity of protein binding to the DNA sequence used.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Andere Bezeichnung: Gata4 (GATA4 Produkte)

Synonyme: gata-4 Protein, xgata4 Protein, xGATA-4 Protein, MGC81427 Protein, GATA4 Protein, GATA-4 Protein, Gata4 Protein, gata4 Protein, ASD2 Protein, VSD1 Protein, Gata-4 Protein, gata Protein, GATA binding protein 4 Protein, GATA binding protein 4 L homeolog Protein, glutamyl-tRNA(Gln) amidotransferase subunit A Protein, gata4 transcription factor Protein, GATA binding protein 4 S homeolog Protein, GATA4 Protein, gata4.L Protein, gata4 Protein, gatA4 Protein, Gata4 Protein, gata4.S Protein

Hintergrund: This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 44.4 kDa

NCBI Accession: NP_002043

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis