LIG1 Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target Alle LIG1 Proteine anzeigen
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses LIG1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.
- Applikation
- Antibody Production (AbP), Standard (STD)
- Produktmerkmale
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- Recombinant human DNA ligase 1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Reinheit
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product LIG1 Protein
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Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-919) protein (GST tag)
LIG1 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919) protein (Strep Tag)Crystallography grade LIG1 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-916) protein (Strep Tag)Crystallography grade LIG1 Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919), (full length) protein (GST tag)LIG1 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Kommentare
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The tag is located at the C-terminal.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Okazaki fragment maturation involves α-segment error editing by the mammalian FEN1/MutSα functional complex." in: The EMBO journal, Vol. 34, Issue 13, pp. 1829-43, (2015) (PubMed).
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: "Okazaki fragment maturation involves α-segment error editing by the mammalian FEN1/MutSα functional complex." in: The EMBO journal, Vol. 34, Issue 13, pp. 1829-43, (2015) (PubMed).
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- Target
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
- Andere Bezeichnung
- Dna Ligase 1 (LIG1 Produkte)
- Synonyme
- ATLIG1 Protein, DNA ligase 1 Protein, T6D22.23 Protein, ligI Protein, AL033288 Protein, LigI Protein, lig1 Protein, wu:fc54a11 Protein, DNA ligase 1 Protein, DNA ligase 1, putative Protein, ligase I, DNA, ATP-dependent L homeolog Protein, ligase I, DNA, ATP-dependent Protein, LIG1 Protein, PB000674.02.0 Protein, PTRG_06243 Protein, PKH_140260 Protein, lig1.L Protein, Lig1 Protein, lig-1 Protein, lig1 Protein
- Hintergrund
- This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.
- Molekulargewicht
- 101.6 kDa
- NCBI Accession
- NP_000225
- Pathways
- Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA
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