DDX5 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2719286

Produktdetails

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DDX5 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human DDX5 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Taniguchi, Iizumi, Watanabe, Masuda, Morita, Aono, Toriyama, Oishi, Goi, Sakai: "Resveratrol directly targets DDX5 resulting in suppression of the mTORC1 pathway in prostate cancer." in: Cell death & disease, Vol. 7, pp. e2211, (2016) (PubMed).

Kost, Yang, Li, Zhang, Liu, Kim, Ahn, Lee, Liu: "A Novel Anti-Cancer Agent, 1-(3,5-Dimethoxyphenyl)-4-[(6-Fluoro-2-Methoxyquinoxalin-3-yl)Aminocarbonyl] Piperazine (RX-5902), Interferes With β-Catenin Function Through Y593 Phospho-p68 RNA Helicase." in: Journal of cellular biochemistry, Vol. 116, Issue 8, pp. 1595-601, (2015) (PubMed).

Antigendetails

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Andere Bezeichnung: Ddx5 (DDX5 Produkte)

Synonyme: G17P1 Protein, HLR1 Protein, HUMP68 Protein, p68 Protein, 2600009A06Rik Protein, Hlr1 Protein, wu:fa56a07 Protein, wu:fb11e01 Protein, wu:fb16c10 Protein, wu:fb53b05 Protein, ddx5 Protein, DEAD-box helicase 5 Protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 Protein, DEAD (Asp-Glu-Ala-Asp) box helicase 5 Protein, DEAD-box helicase 5 L homeolog Protein, DDX5 Protein, Ddx5 Protein, ddx5 Protein, ddx5.L Protein

Hintergrund: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 69 kDa

NCBI Accession: NP_004387

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus