GDF11 Protein

Produktnummer ABIN2715289

Produktdetails

Target: GDF11 (Growth Differentiation Factor 11 (GDF11))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human BMP11 / GDF11 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin-Niveau: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 was determined by its ability to inhibit induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.08-0.10 ug/ml.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Sterile filtered through 0.2 μM filtered, Lyophilized with no additives

Konservierungsmittel: Without preservative

Handhabung: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1~1.0 mg/mL, and keep pH at acidic condition below 5.0 Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (e.g. 0.1 % BSA) and store in working aliquots at -20 °C to -80 °C

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: GDF11 (Growth Differentiation Factor 11 (GDF11))

Andere Bezeichnung: Bmp11,gdf11 (GDF11 Produkte)

Synonyme: BMP-11 Protein, BMP11 Protein, Bmp11 Protein, GDF11 Protein, growth differentiation factor 11 Protein, GDF11 Protein, Gdf11 Protein, gdf11 Protein

Hintergrund: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein plays a role in the development of the nervous and other organ systems, and may regulate aging.

Molekulargewicht: 25 kDa

NCBI Accession: NP_005802