Axin Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2715088

Produktdetails

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Axin Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Axin-1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Referenzen

Cha, Cho, Lee, Lee, Jeong, Moon, Yun, Yang, Choi, Yoon, Kim, Kim, Kaduwal, Lee, Min, Kim, Han, Choi: "Small-molecule binding of the axin RGS domain promotes β-catenin and Ras degradation." in: Nature chemical biology, Vol. 12, Issue 8, pp. 593-600, (2016) (PubMed).

Antigendetails

Target: Axin (AXIN1) (Axin 1 (AXIN1))

Andere Bezeichnung: Axin-1 (AXIN1 Produkte)

Synonyme: AXIN1 Protein, axin Protein, fused Protein, xAxin Protein, LOC100226467 Protein, AXIN Protein, PPP1R49 Protein, AI316800 Protein, Axin Protein, Fu Protein, Kb Protein, Ki Protein, kinky Protein, knobbly Protein, AXIN1-RGS Protein, wu:fi22e03 Protein, wu:fj14c08 Protein, wu:fk11b03 Protein, zgc:109872 Protein, axin 1 Protein, axin 1 S homeolog Protein, axin 1 L homeolog Protein, AXIN1 Protein, axin1.S Protein, axin1.L Protein, Axin1 Protein, axin1 Protein

Hintergrund: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 91.5 kDa

NCBI Accession: NP_851393

Pathways: WNT Signalweg, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling