ARG2 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2714866

Produktdetails

Target: ARG2 (Arginase, Type II (ARG2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ARG2 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Arginase-2 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: L-Arginase activity verified in a biochemical assay : Arginase 2 (ARG2, ) activity was measured in a colorimetric biochemical assay. Arginase 1 catalyzes the conversion of arginine to ornithine and urea. After incubation of the protein in a solution containing arginine, the reaction is stopped, and the urea concentration is measured by a chemical reaction that produces a colored product that absorbs at 430 nm. By measuring the absorbance at 430 nm and comparing to a standard, the specific activity of this preparation of ARG2 was calculated to be approximately 10U/mg. Unit definition: 1 unit of ARG2 converts 1 μmole of L-arginine to ornithine and urea per minute at pH 9.5 and 37°C.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: ARG2 (Arginase, Type II (ARG2))

Andere Bezeichnung: Arginase-2 (ARG2 Produkte)

Synonyme: AII Protein, AU022422 Protein, zgc:65793 Protein, zgc:85630 Protein, wu:fb67g02 Protein, ARG2 Protein, LeARG2 Protein, arg1 Protein, arg3 Protein, arg2 Protein, arg2-c Protein, arg2-b Protein, arginase 2 Protein, arginase type II Protein, arginase 2 L homeolog Protein, arginase 2 S homeolog Protein, ARG2 Protein, Arg2 Protein, arg2 Protein, arg2.L Protein, arg2.S Protein

Hintergrund: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described.

Molekulargewicht: 36 kDa

NCBI Accession: NP_001163