ATG10 Protein (Transcript Variant 3) (His tag)
Kurzübersicht für ATG10 Protein (Transcript Variant 3) (His tag) (ABIN2714791)
Target
Alle ATG10 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- Transcript Variant 3
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Aufreinigungstag / Konjugat
- Dieses ATG10 Protein ist gelabelt mit His tag.
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Produktmerkmale
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- Recombinant human APG10L / ATG10 (transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin-Niveau
- < 0.1 EU per μg protein as determined by LAL test
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Autophagy Related 10 (ATG10) (AA 1-215) protein (His tag)
custom-made ATG10 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)custom-made ATG10 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (GST tag)ATG10 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy Related 10 (ATG10) (AA 1-220) protein (His-IF2DI Tag)ATG10 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)ATG10 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % SDS
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Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Kommentare
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The tag is located at the C-terminal.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 50 μg/mL
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Buffer
- 20 mM Phosphate buffer, 150 mM NaCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Lagerung
- -80 °C
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Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ATG10 (Autophagy Related 10 (ATG10))
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Andere Bezeichnung
- Apg10l,atg10
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Hintergrund
- Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
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Molekulargewicht
- 24.5 kDa
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NCBI Accession
- NP_001124500
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Pathways
- ER-Nucleus Signaling, Autophagie
Target
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