PRKG1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2713946

Produktdetails

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Protein-Typ: Recombinant

Proteineigenschaft: Transcript Variant 2

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses PRKG1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human CGK1 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Andere Bezeichnung: Cgk1 (PRKG1 Produkte)

Synonyme: prkg1 Protein, zgc:63955 Protein, Pkgi Protein, cGk1 Protein, 1 Protein, PKG Protein, PRKG1B Protein, PRKGR1B Protein, cGK Protein, cGK 1 Protein, cGK1 Protein, cGKI Protein, cGKI-BETA Protein, cGKI-alpha Protein, CGKI Protein, cGKI-beta Protein, AW125416 Protein, Gm19690 Protein, Prkg1b Protein, Prkgr1b Protein, Prkg1beta Protein, protein kinase, cGMP-dependent, type Ia Protein, protein kinase, cGMP-dependent, type I Protein, protein kinase, cGMP-dependent, type 1 Protein, prkg1a Protein, PRKG1 Protein, prkg1 Protein, Prkg1 Protein

Hintergrund: Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity.

Molekulargewicht: 77.6 kDa

NCBI Accession: NP_006249