Band 3/AE1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2713525

Produktdetails

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Band 3/AE1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human CD233 / SLC4A1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Band 3/AE1 (SLC4A1) (Solute Carrier Family 4, Anion Exchanger, Member 1 (erythrocyte Membrane Protein Band 3, Diego Blood Group) (SLC4A1))

Andere Bezeichnung: Cd233,slc4a1 (SLC4A1 Produkte)

Synonyme: AE1 Protein, BND3 Protein, CD233 Protein, DI Protein, EMPB3 Protein, EPB3 Protein, FR Protein, RTA1A Protein, SW Protein, WD Protein, WD1 Protein, WR Protein, Ae1 Protein, Empb3 Protein, l11Jus51 Protein, ae1 Protein, band3 Protein, MGC152771 Protein, zgc:111889 Protein, zgc:152771 Protein, si:dz180g5.1 Protein, LOC100136769 Protein, slc4a1 Protein, wd1 Protein, bnd3 Protein, epb3 Protein, cd233 Protein, empb3 Protein, rta1a Protein, MGC80391 Protein, SLC4A1 Protein, BB3 Protein, EAT Protein, solute carrier family 4 member 1 (Diego blood group) Protein, solute carrier family 4 (anion exchanger), member 1 Protein, solute carrier family 4 (anion exchanger), member 1a (Diego blood group) Protein, Band 3 Protein, erythrocyte membrane protein band 4.1 like 3 Protein, solute carrier family 4 member 1 (Diego blood group) L homeolog Protein, solute carrier family 4 member 1 Protein, SLC4A1 Protein, Slc4a1 Protein, slc4a1a Protein, LOC100136769 Protein, EPB41L3 Protein, slc4a1.L Protein, slc4a1 Protein

Hintergrund: The protein encoded by this gene is part of the anion exchanger (AE) family and is expressed in the erythrocyte plasma membrane, where it functions as a chloride/bicarbonate exchanger involved in carbon dioxide transport from tissues to lungs. The protein comprises two domains that are structurally and functionally distinct. The N-terminal 40 kDa domain is located in the cytoplasm and acts as an attachment site for the red cell skeleton by binding ankyrin. The glycosylated C-terminal membrane-associated domain contains 12-14 membrane spanning segments and carries out the stilbene disulphonate-sensitive exchange transport of anions. The cytoplasmic tail at the extreme C-terminus of the membrane domain binds carbonic anhydrase II. The encoded protein associates with the red cell membrane protein glycophorin A and this association promotes the correct folding and translocation of the exchanger. This protein is predominantly dimeric but forms tetramers in the presence of ankyrin. Many mutations in this gene are known in man, and these mutations can lead to two types of disease: destabilization of red cell membrane leading to hereditary spherocytosis, and defective kidney acid secretion leading to distal renal tubular acidosis. Other mutations that do not give rise to disease result in novel blood group antigens, which form the Diego blood group system. Southeast Asian ovalocytosis (SAO, Melanesian ovalocytosis) results from the heterozygous presence of a deletion in the encoded protein and is common in areas where Plasmodium falciparum malaria is endemic. One null mutation in this gene is known, resulting in very severe anemia and nephrocalcinosis.

Molekulargewicht: 101.6 kDa

NCBI Accession: NP_000333