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LAP3 Protein (Myc-DYKDDDDK Tag)

LAP3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
Produktnummer ABIN2712411
  • Target Alle LAP3 Proteine anzeigen
    LAP3 (Cytosol Aminopeptidase (LAP3))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    • 6
    • 1
    • 1
    • 1
    Human
    Quelle
    • 3
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Aufreinigungstag / Konjugat
    Dieses LAP3 Protein ist gelabelt mit Myc-DYKDDDDK Tag.
    Applikation
    Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
    Spezifität
    Optimal preservation of protein structure, post-translational modifications and functions.
    Produktmerkmale
    • Recombinant human Cytosol aminopeptidase protein expressed in HEK293 cells.
    • Produced with end-sequenced ORF clone
    • Tested for bioactivity.
    Reinheit
    > 80 % as determined by SDS-PAGE and Coomassie blue staining
    Biological Activity Comment
    LAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, ) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 μl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours.
    Top Product
    Discover our top product LAP3 Protein
  • Applikationshinweise
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Kommentare

    The tag is located at the C-terminal.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    > 50 μg/mL
    Buffer
    25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    LAP3 (Cytosol Aminopeptidase (LAP3))
    Andere Bezeichnung
    Cytosol Aminopeptidase (LAP3 Produkte)
    Synonyme
    LAP Protein, LAPEP Protein, PEPS Protein, 2410015L10Rik Protein, AA410100 Protein, LAP-3 Protein, Lap Protein, Lapep Protein, Pep-7 Protein, Pep-S Protein, Pep7 Protein, Peps Protein, cytosol aminopeptidase Protein, leucine aminopeptidase 3 Protein, pepA Protein, Plabr_1567 Protein, Dester_0800 Protein, Weevi_0186 Protein, Marky_0570 Protein, Halhy_2623 Protein, FsymDg_3217 Protein, Flexsi_2186 Protein, Runsl_3980 Protein, lap3 Protein, LAP3 Protein, Lap3 Protein
    Hintergrund
    Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. [UniProtKB/Swiss-Prot Function]
    Molekulargewicht
    56 kDa
    NCBI Accession
    NP_056991
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