DOT1L Protein (AA 1-416) (GST tag)

Produktnummer ABIN2669718

Produktdetails

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-416

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses DOT1L Protein ist gelabelt mit GST tag.

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktmerkmale: Recombinant DOT1L (1-416), an active truncation that includes amino acids 1-416 (accession number NP_115871.1) ,was expressed in E. coli cells and contains an N-terminal GST tag with an observed molecular weight of 72 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant DOT1L (1-416), active protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K79 methyltransferase HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 30 ng/μl Recombinant Nucleosomes, 30 ng/μl DOT1L (1-416 aa) at 2 hours at room temperature. Activity was detected by fluorography.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Andere Bezeichnung: DOT1L (DOT1L Produkte)

Synonyme: NV11307 Protein, DOT1L Protein, DOT1 Protein, KMT4 Protein, A630076O07 Protein, AW907654 Protein, Dot1 Protein, mDot1 Protein, histone-lysine N-methyltransferase, H3 lysine-79 specific Protein, DOT1 like histone lysine methyltransferase Protein, histone methyltransferase Protein, DOT1-like, histone H3 methyltransferase (S. cerevisiae) Protein, LOC100116648 Protein, DOT1L Protein, DOT1 Protein, Dot1l Protein

Hintergrund: DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.

Molekulargewicht: 72 kDa