KDM6A Protein (DYKDDDDK Tag)

Produktnummer ABIN2669708

Produktdetails

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Aufreinigungstag / Konjugat: Dieses KDM6A Protein ist gelabelt mit DYKDDDDK Tag.

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktmerkmale: Recombinant UTX / KDM6A (accession number NP_066963.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 158.8 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant UTX / KDM6A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K27me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant UTX / KDM6A protein, and 3.3 μM H3K27me3 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: KDM6A (Lysine (K)-Specific Demethylase 6A (KDM6A))

Andere Bezeichnung: UTX / KDM6A (KDM6A Produkte)

Synonyme: KABUK2 Protein, UTX Protein, bA386N14.2 Protein, Utx Protein, si:ch211-222m21.1 Protein, utx Protein, kdm6a Protein, tutx Protein, uty Protein, xtutx Protein, lysine demethylase 6A Protein, lysine-specific demethylase 6A Protein, lysine (K)-specific demethylase 6A Protein, lysine demethylase 6A S homeolog Protein, KDM6A Protein, LOC100539898 Protein, Kdm6a Protein, kdm6a Protein, kdm6a.S Protein

Hintergrund: KDM6A (lysine (K)-specific demethylase 6A), also known as UTX (Ubiquitously Transcribed Tetratricopeptide Repeat, X Chromosome) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 27 residues of histone H3, while it has no activity on monomethylated H3K27 residues. KDM6A plays a central role in posterior development by regulating HOX gene expression. Demethylation of lysine 27 is concomitant with methylation of lysine 4 of histone H3 and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.

Molekulargewicht: 158.8 kDa

Pathways: Tube Formation, Warburg Effekt