JMJD2D Protein (DYKDDDDK Tag)
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- Target Alle JMJD2D (KDM4D) Proteine anzeigen
- JMJD2D (KDM4D) (Lysine (K)-Specific Demethylase 4D (KDM4D))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Baculovirus
- Aufreinigungstag / Konjugat
- Dieses JMJD2D Protein ist gelabelt mit DYKDDDDK Tag.
- Applikation
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Produktmerkmale
- Recombinant JMJD2D / KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 63.2 kDa.
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Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-523) protein (GST tag)
KDM4D Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-523) protein (Strep Tag)Crystallography grade KDM4D Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Lysine (K)-Specific Demethylase 4D (KDM4D) (AA 1-510) protein (Strep Tag)Crystallography grade KDM4D Spezies: Maus Wirt: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Applikationshinweise
- Recombinant JMJD2D / KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me3 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant JMJD2D / KDM4D protein, and 3.3 μM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- JMJD2D (KDM4D) (Lysine (K)-Specific Demethylase 4D (KDM4D))
- Andere Bezeichnung
- JMJD2D / KDM4D (KDM4D Produkte)
- Synonyme
- JMJD2D Protein, 4932416A15 Protein, Jmjd2d Protein, KDM4D Protein, lysine demethylase 4D Protein, lysine-specific demethylase 4D Protein, lysine (K)-specific demethylase 4D Protein, KDM4D Protein, LOC524768 Protein, Kdm4d Protein, LOC617971 Protein, LOC100067461 Protein, LOC698455 Protein, LOC100514356 Protein
- Hintergrund
- KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
- Molekulargewicht
- 63.2 kDa
- Pathways
- Warburg Effekt
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