Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa.
Crystallography grade
PRMT5
Spezies: Human
Wirt: Insektenzellen
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, ELISA, SDS, Crys
Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methyltransferase. HMT Assay Conditions:50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl H3 (1-21) peptide, 20 ng/μl Recombinant PRMT5 protein at 2 hours at room temperature. Activity was detected by fluorography.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Lagerung
-80 °C
Informationen zur Lagerung
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
HRMT1L5 Protein, IBP72 Protein, JBP1 Protein, SKB1 Protein, SKB1Hs Protein, Jbp1 Protein, Skb1 Protein, si:zc14a17.2 Protein, skb1 Protein, wu:fb95f10 Protein, zgc:110669 Protein, hsl7 Protein, DDBDRAFT_0187422 Protein, DDBDRAFT_0235403 Protein, DDB_0187422 Protein, DDB_0235403 Protein, NV18830 Protein, jbp1 Protein, ibp72 Protein, skb1hs Protein, hrmt1l5 Protein, protein arginine methyltransferase 5 Protein, protein arginine N-methyltransferase 5 Protein, protein arginine methyltransferase 5 L homeolog Protein, putative arginine N-methyltransferase, type II Protein, Skb1 family protein Protein, Protein arginine N-methyltransferase 5 Protein, PRMT5 Protein, Prmt5 Protein, prmt5 Protein, prmt5.L Protein, LOC100115766 Protein, prmt-5 Protein
Hintergrund
PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.