C-Type Lectin Domain Family 1, Member B (CLEC1B) (AA 157-229), (C-Term) (Active) protein (Fc Tag)

Produktnummer ABIN2666791

Produktdetails

Target: C-Type Lectin Domain Family 1, Member B (CLEC1B)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 157-229, C-Term

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Fc Tag

Applikation: Flow Cytometry (FACS), Intracellular Flow Cytometry (ICFC)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1.0 EU per μg of protein as determine by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Recombinant mouse CLEC2, when coated on a plate (2 μg/ml, 100 μl/well) is able to bind biotinylated mouse GP38 in a dose dependent manner with an apparent kD <5.0 nM.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Konzentration: 200 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: C-Type Lectin Domain Family 1, Member B (CLEC1B)

Andere Bezeichnung: CLEC2 (CLEC1B Produkte)

Synonyme: 1810061I13Rik Protein, CLEC2 Protein, CLEC2B Protein, PRO1384 Protein, QDED721 Protein, Clec-2 Protein, Clec2 Protein, CLEC1B Protein, RGD1563517 Protein, CLEC9A Protein, C-type lectin domain family 1 member B Protein, C-type lectin domain family 1, member b Protein, C-type lectin domain family 1, member B Protein, CLEC1B Protein, Clec1b Protein

Substanzklasse: Viral Protein

Hintergrund: CLEC2, also termed CLEC-1B, is a C-type lectin-like receptor. CLEC2 is highly expressed on resting platelets and megakaryocytes, and expressed at lower levels on several hematopoietic cells including monocytes, macrophages, dendritic cells, and granulocytes. The first ligand identified for CLEC2 was rhodocytin, snake venom toxin. Rhodocytin is a strong platelet activator, and this effect is mediated by CLEC2, which has a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (ITAM). Exposure of platelets to rhodocytin leads to tyrosine phosphorylation of the ITAM-like motif and Syk-dependent platelet activation. Podoplanin, also termed GP38 is an endogenous ligand of CLEC2. This molecule was found to be expressed on the surface of tumor cells and facilitates tumor metastasis by inducing platelet aggregation. Podoplanin is also expressed on endothelial cells, and mice deficient in this protein show abnormal patterns of lymphatic vessel formation. In addition, CLEC2 functions as an activating receptor on monoyctes and neutrophils to induce phagocytosis and proinflammatory cytokine production. CLEC2 enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. Also, CLEC2 modulates the capacity of T cells to home to lymph nodes and stimulates natural killer cell mediated cytotoxicity.

Molekulargewicht: The 409 amino acid recombinant protein has a predicted molecular mass of approximately 46.7 kDa. The protein migrates at about 60 kDa by SDS-PAGE in DTT-reducing conditions and about 120 kDa in non-reducing conditions. The predicted N-terminal amino acid