CCL27 Protein (AA 25-112)

Produktnummer ABIN2666614

Produktdetails

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 25-112

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Immunofluorescence (IF)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract BaF3-hCCR10 transfectants in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CCL27 (Chemokine (C-C Motif) Ligand 27 (CCL27))

Andere Bezeichnung: CCL27 (CCL27 Produkte)

Synonyme: ALP Protein, ILC Protein, CTACK Protein, ESkine Protein, CCL27 Protein, CTAK Protein, ESKINE Protein, PESKY Protein, SCYA27 Protein, AW558992 Protein, Ccl27 Protein, Scya27 Protein, Scya27a Protein, C-C motif chemokine ligand 27 Protein, uncharacterized LOC508933 Protein, chemokine (C-C motif) ligand 27A Protein, Ccl27 Protein, CCL27 Protein, LOC508933 Protein, Ccl27a Protein

Hintergrund: CCL27 is highly expressed in skin where it is constitutively produced by keratinocytes. CCL27 binds to CCR10 (shared with CCL28) which is expressed on melanocytes, fibroblasts, and dermal microvascular endothelial cells. The interaction of CCL27 with CCR10 has a main role in skin homeostasis and inflammation. It has been described that more than 90 % of the lymphocytes in the skin of patients with psoriasis, atopic dermatitis, and allergic-contact dermatitis express CCR10. In melanoma, CCR10 increases the ability of neoplastic cells to grow, invade tissues, disseminate to lymph nodes, and escape host immune responses. Also, it has been described that human skin tumors elude T cell-mediated antitumor immune responses by down-regulating the expression of CCL27. Interestingly, this down-regulation is mediated by EGFR signaling. In addition, CCL27 upregulation in melanoma cells impairs tumor growth due to the recruitment and accumulation of T cells and natural killer cells at tumor sites, resulting in enhanced antitumor immune responses.

Molekulargewicht: The 88 amino acid recombinant protein has a predicted molecular mass of approximately 10 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein migrates at 12 kDa by SDS-PAGE. The N-terminal amino acid is Phe.