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IL1RL1 Protein (AA 19-328)

IL1RL1 Spezies: Human Wirt: HEK-293 Cells Recombinant > 95 % , as determined by Coomassie stained SDS-PAGE. FACS, IF Active
Produktnummer ABIN2666553
  • Target Alle IL1RL1 Proteine anzeigen
    IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 19-328
    Spezies
    • 21
    • 11
    Human
    Quelle
    • 15
    • 5
    • 5
    • 4
    • 2
    • 1
    HEK-293 Cells
    Applikation
    Flow Cytometry (FACS), Immunofluorescence (IF)
    Reinheit
    > 95 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

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  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: ED50 = 15 - 90 ng/ml, corresponding to a specific activity of 1.1 - 6.6 x 104 units/mg , as determined by inhibition of D10.G4.1 cell proliferation induced by IL-33.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
    Buffer
    0.22 μm filtered protein solution is in 10 mM NaHPO4, 0.3M NaCl, and pH 7.2.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
  • Target
    IL1RL1 (Interleukin 1 Receptor-Like 1 (IL1RL1))
    Andere Bezeichnung
    IL-1RL1 (IL1RL1 Produkte)
    Synonyme
    DER4 Protein, Fit-1 Protein, Ly84 Protein, ST2L Protein, St2 Protein, St2-rs1 Protein, T1 Protein, T1/ST2 Protein, FIT-1 Protein, IL33R Protein, ST2 Protein, ST2V Protein, FIT1 Protein, interleukin 1 receptor-like 1 Protein, interleukin 1 receptor like 1 Protein, Il1rl1 Protein, IL1RL1 Protein
    Hintergrund
    IL-1RL1/ST2 was initially identified in stimulated Balc3T3 cells and is encoded by the IL-1RL1 gene that produces different isoforms by alternative splicing. In humans, four isoforms have been described: a membrane attached ST2L isoform and a soluble protein (sST2/ IL1RL-a) that lacks the transmembrane and cytoplasmic domains, which acts as a natural antagonist of IL-33. The other splice variants are ST2V and ST2LV. ST2V lacks the third immunoglobulin motif and C-terminal portion of ST2 and is mainly present in the gut whereas ST2LV does not have the transmembrane domain. The membrane isoform of ST2L (IL-1RL1) associates with IL-1 receptor accessory proteins (IL-1RAcP) to form the IL-33R. The interaction of IL-33 to its receptor activates NF-kB and MAP kinases and induces Th2 cytokines from in vitro polarized Th2 cells. In vivo, IL-33 promotes the expression of IL-4, IL-5, and IL-13. IL-33 acts as an inflammatory cytokine in Th2-type immune responses during asthma and atopic dermatitis. It is host-protective against helminth infections and reduces atherosclerosis by promoting Th2-type immune responses. Human aortic, coronary artery, and heart microvascular endothelial cells express mRNA for ST2L and sST2 isoforms, acting as a source for sST2 proteins. In fact, sST2 is considered as a prognostic biomarker in myocardial infarction and heart failure. In addition, sST2 has been associated with other human diseases including asthma with acute exacerbation, eosinophilic pneumonia, sepsis and trauma, and exacerbated idiopathic pulmonary fibrosis.
    Molekulargewicht
    The 550 amino acid recombinant protein has a predicted molecular mass of approximately 62 kDa. The DTT-reduced and non-reduced protein migrate approximately at 100 kDa and 200 kDa by SDS-PAGE. The predicted N-terminal amino acid is Lys.
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