Tumor Necrosis Factor (TNF) (Active) protein (Biotin)

Details zu Produkt Nr. ABIN2566977, Anbieter: Anmelden zum Anzeigen
Proteinname
  • DIF
  • TNF-alpha
  • TNFA
  • TNFSF2
  • RATTNF
  • Tnfa
  • tnf
  • TNF-a
  • TNFalpha
  • Tnfsf1a
  • TNFa
  • cTNF
  • Tnf-alpha
  • tnfa-like
  • TNF-ALPHA
  • dif
  • tnfa
  • xtnf
  • tnfsf2
  • tnf-alpha
  • Cachectin
  • tumor necrosis factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • lipopolysaccharide induced TNF factor
  • tumor necrosis factor a (TNF superfamily, member 2)
  • TNF
  • Tnf
  • tnf
  • tnfb
  • tnf-alpha
  • LITAF
  • LOC103694380
  • tnfa
Spezies
Human
115
53
29
9
6
5
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
HEK-293 Cells
160
26
19
6
4
3
3
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses Tumor Necrosis Factor Protein ist gelabelt mit Biotin.
Applikation
ELISA, Western Blotting (WB)
Optionen
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Produktmerkmale

Measured by its binding ability in a functional ELISA. Immobilized Human TNFR1/CD120a Protein,C-Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha Protein . The EC50 is typically 2. 8-11. 4 ng/mL.

Reinheit >95 % as determined by reduced SDS-PAGE.
Hintergrund Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated. The primary amine of Biotinylated rh TNFawas chemically labeled by specially optimized long spacer biotin reagent without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin reagent with optimized long spacer provides improved biotin exposure and less steric hindrance,thus giving superior sensitivity and response when detection with streptavidin. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.
Molekulargewicht 17.4 kDa
Gen-ID 7124
NCBI Accession NP_000585
UniProt P01375
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Applikationshinweise This recombinant protein can be used for WB, ELISA. For research use only.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
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