Interleukin 1 alpha (IL1A) (Active) protein (Biotin)

Details zu Produkt Nr. ABIN2566705, Anbieter: Anmelden zum Anzeigen
Proteinname
  • Il-1
  • Il-1a
  • IL-1 alpha
  • IL-1A
  • IL1
  • IL1-ALPHA
  • IL1F1
  • IL-1alpha
  • L1A
  • interleukin 1 complex
  • interleukin 1 alpha
  • Il1
  • Il1a
  • IL1A
Spezies
Human
58
29
12
5
4
3
3
3
3
2
2
2
1
1
1
1
1
Quelle
HEK-293 Cells
100
13
4
2
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses IL1A Protein ist gelabelt mit Biotin.
Applikation
ELISA, Western Blotting (WB)
Optionen
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Produktmerkmale

Measured by its binding ability in a functional ELISA. Immobilized Human IL1R2 /CD121b Protein,C-Fc Tag at 10 μg/mL (100 μL/well),can bind Biotinylated Human IL1a Protein,HEK293 Expressed. The EC50 is typically 0. 2-2 μg/mL.

Reinheit >97 % as determined by SDS-PAGE.
Hintergrund Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes. The primary amine of rh IL1a was labeled by biotin without decreasing its bioactivity, and has been used successfully for easy detection in ELISA, dot blot or Western blot, immunohistochemistry applications using streptavidin or avidin-conjugated probes. Biotin and other reactive related chemicals have been removed thoroughly by desalting after reaction termination.
Molekulargewicht 18 kDa
Gen-ID 3552
NCBI Accession NP_000566
UniProt P01583
Pathways NF-kappaB Signalweg, Autophagie
Applikationshinweise This recombinant protein can be used for WB, ELISA. For research use only.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
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