PCNA Protein

Produktnummer ABIN2452200

Produktdetails

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Dot Blot (DB), Functional Studies (Func)

Produktmerkmale: Source: Human PCNA was over-expressed in E. coli as a recombinant full-size protein without any tag and highly purified.

Reinheit: > 98 % purity as determined by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: 1. Functional studies on DNA replication,recombination and repair.
2. Identification of proteins interacting with PCNA by using PCNA-conjugated resin.
3. Ubiquitination targets.
4. SDS-PAGE
5. Western blot
6. Dot blot.
7. ELISA.
Not tested for other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: 25 mM HEPES ( pH 7.9), 1 mM EDTA, 0.01 % NP40, 1 mM DTT, 2 g/mL leupeptin, 0.1 mM PMSF, 75 mM NaCl, 50 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Upon arrival spin-down and store at -20 C (or at -80 C for longer storage)

Antigendetails

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA (PCNA Produkte)

Synonyme: PCNAR Protein, Pcna/cyclin Protein, pcna Protein, AO090003000846 Protein, 53/13 Protein, CG9193 Protein, DmPCNA Protein, Dmel\\CG9193 Protein, MUS209 Protein, Mus209 Protein, PCNA Protein, PCNA/mus209 Protein, Pcna Protein, dPCNA Protein, l(2)02448 Protein, l(2)56F Protein, l(2)56Fa Protein, l(2)s1534 Protein, cb16 Protein, fb36g03 Protein, wu:fa28e03 Protein, wu:fb36g03 Protein, pcna-A Protein, proliferating cell nuclear antigen Protein, DNA polymerase III sliding clamp Protein, Proliferating cell nuclear antigen Protein, Proliferating cell nuclear antigen-like Protein, proliferating cell nuclear antigen L homeolog Protein, proliferating cell nuclear antigen 1 Protein, PCNA Protein, Pcna Protein, pcna Protein, MMP_RS08810 Protein, ANI_1_512014 Protein, AOR_1_1500154 Protein, MGC53867 Protein, pcna.L Protein, pcna1 Protein, LOC106420145 Protein, pcn1 Protein

Hintergrund: PCNA (Proliferating cell nuclear antigen) is a homotrimeric protein (261 aa, 29 kDa) known to act as a co-factor for DNA polymerase δ, which is responsible for leading strand DNA replication. PCNA was originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. Crystal structure data suggests that a PCNA homotrimer ring encircles and slides along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associates with DNA, thus facilitating rapid processing of DNA. PCNA is a useful marker for DNA synthesis and some cancers. It is highly conserved among most amimals.

UniProt: P12004, P04961, P17918, Q9PTP1

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie