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Diphtheria Toxin Protein

Spezies: Corynebacterium diphtheriae Wirt: Corynebacterium diphtheriae Native > 95 % purity by SDS-PAGE (see below, SDS-PAGE without mercaptoethanol) ELISA, WB, Inhibition, Func, NC, SDS Active
Produktnummer ABIN2452169
  • Target Alle Diphtheria Toxin Produkte
    Diphtheria Toxin
    Protein-Typ
    Native
    Biologische Aktivität
    Active
    Spezies
    Corynebacterium diphtheriae
    Quelle
    • 1
    Corynebacterium diphtheriae
    Applikation
    ELISA, Western Blotting (WB), Blocking Antibody (Inhibition), Functional Studies (Func), Negative Control (NC), SDS-PAGE (SDS)
    Reinheit
    > 95 % purity by SDS-PAGE (see below, SDS-PAGE without mercaptoethanol)
  • Applikationshinweise
    1) Inhibition of protein synthesis in eucaryotic cells
    2) Negative selection agent for ES cells in construction of transgenic mouse
    3) Putative drug for treatment of malignant tumors such as leukemia
    4) Antigen for Western blotting and ELISA
    5) As a marker of Diphtheria toxin in SDS-PAGE
    Kommentare

    Activity test: Addition of 20 ~30 pg/mL of Diphtheria toxin in growth medium caused 50 % lethality after 43 h in Vero cells. Nicking of Diphtheria toxin by trypsin by trypsin and reduction by dithiothreitol further increase the activity

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    5 mg/mL
    Buffer
    20 mM Tris-HCl ( pH 7.2), 150 mM NaCl
    Lagerung
    -80 °C
  • Yagi, Nada, Watanabe, Tamemoto, Kohmura, Ikawa, Aizawa: "A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene." in: Analytical biochemistry, Vol. 214, Issue 1, pp. 77-86, (1994) (PubMed).

    Pappenheimer: "Diphtheria toxin." in: Annual review of biochemistry, Vol. 46, pp. 69-94, (1977) (PubMed).

    Uchida, Pappenheimer, Harper: "Diphtheria toxin and related proteins. 3. Reconstitution of hybrid "diphtheria toxin" from nontoxic mutant proteins." in: The Journal of biological chemistry, Vol. 248, Issue 11, pp. 3851-4, (1973) (PubMed).

  • Target
    Diphtheria Toxin
    Abstract
    Diphtheria Toxin Produkte
    Hintergrund
    This Diphtheria toxin was highly purified from the growth media of Corynebacterium diphtheriae strain PW8 as mostly unnicked form. Diphtheria toxin is a single polypeptide chain of 535 amino acids (58 kD) and nicked by cellular protease like furin to give fragments A (N-terminal, 21 kDa) and B (C-terminal, 37 kDa) which are linked by disulfide bridges. Binding to the cell surface of frgment B allows fragment A to penetrate the host cell. Fragment A catalyzes the ADP-ribosylation of eucaryotic elongation factor-2 (eEF2) by using NAD as a substrate, thus inactivating eEF2 and inhibiting protein synthesis.
    UniProt
    Q5PY51
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