Dermonecrotizing Toxin (Active) Protein

Details zu Produkt Nr. ABIN2452168, Anbieter: Anmelden zum Anzeigen
Proteinname
Spezies
Bordetella bronchiseptica
Quelle
Bordetella bronochiseptica
Protein-Typ
Native
Biologische Aktivität
Active
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Produktmerkmale Activity: DNT at 5 ng/mL induces ballooning morphological change in cultured mouse osteoblastic cell line MC3T3-E1.
Reinheit > 95 % purity by SDS-PAGE (CBB staining)
Sterilität Sterile filtered
Hintergrund Dermonecrotic toxin (DNT) is a common toxin produced by pathogenic bacteria belonging to the genus Bordetella, such as B. pertussis, B. parapertussis, and B. bronchiseptica. This toxin activates small GTP-binding proteins such as Rho, Rac and Cdc42 through deamidation or polyamination for downstream signaling pathways. DNT was purified from B. bronchiseptica strain S798 by propriety chromatography and differential precipitation method.
UniProt Q7WGE1
Applikationshinweise 1) For the studies of the signaling pathways mediated by small GTP-binding proteins.
2) Antigen to be used for detection of infection of Bordetella by immunological method.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.53 mg/mL
Buffer 50 mM sodium phosphate buffer ( pH 7.2), 1 M urea, 0.3 M sodium sulfate
Handhabung Do not freeze!
Lagerung 4 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 2)
SDS-PAGE (SDS) image for Dermonecrotizing Toxin (Active) protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 3)
Western Blotting (WB) image for Dermonecrotizing Toxin (Active) protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 4)
Cell Culture (CC) image for Dermonecrotizing Toxin (Active) protein (ABIN2452168) Dermonecrotizing Toxin (Active) protein (Image 5)
Produkt verwendet in: Masuda, Betancourt, Matsuzawa, Kashimoto, Takao, Shimonishi, Horiguchi: "Activation of rho through a cross-link with polyamines catalyzed by Bordetella dermonecrotizing toxin." in: The EMBO journal, Vol. 19, Issue 4, pp. 521-30, 2000 (PubMed).

Horiguchi, Nakai, Kume: "Simplified procedure for purification of Bordetella bronchiseptica dermonecrotic toxin." in: FEMS microbiology letters, Vol. 54, Issue 1-3, pp. 39-43, 1990 (PubMed).