Interleukin 2 (IL2) (AA 21-153) (Active) Protein

Details zu Produkt Nr. ABIN2181303, Anbieter: Anmelden zum Anzeigen
Proteinname
  • IL2
  • IL-2
  • il2
  • TCGF
  • lymphokine
  • Il-2
  • POIL2
  • interleukin 2
  • interleukin-15
  • IL2
  • il2
  • LOC100303688
  • Il2
Proteineigenschaft
AA 21-153
17
15
10
9
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
76
39
23
5
5
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
HEK-293 Cells
119
26
8
4
2
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Marke ActiveMax®
Produktmerkmale This protein carries no "tag". The protein has a calculated MW of 15.4 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Reinheit >95 % as determined by SDS-PAGE.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Biological Activity Comment Biological Activity: The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse CTLL-2 cells. The ED50<0.1ng/mL, corresponding to a specific activity of >1X107 Unit/mg
Hintergrund Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56 % and 66 % aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
Molekulargewicht 15.4 kDa
UniProt P60568
Pathways JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 5.0
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
Bilder des Herstellers
SDS-PAGE (SDS) image for Interleukin 2 (IL2) (AA 21-153) (Active) protein (ABIN2181303) Human IL-2 on SDS-PAGE under reducing (R) condition. The gel was stained overnight wi...
Binding Studies (Bind) image for Interleukin 2 (IL2) (AA 21-153) (Active) protein (ABIN2181303) Immobilized Human IL-2 R alpha, Fc Tag with a linear range of 0.2-1.56 ng/mL.
Binding Studies (Bind) image for Interleukin 2 (IL2) (AA 21-153) (Active) protein (ABIN2181303) Immobilized Human IL-2, Tag Free with a linear range of 0.625-5 μg/mL.