Fibronectin Protein

Details zu Produkt Nr. ABIN2129270
Proteinname
  • FN
  • cig
  • fibronectin
  • finc
  • lets
  • msf
  • CIG
  • ED-B
  • FINC
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • E330027I09
  • Fn
  • Fn-1
  • FIBNEC
  • fn-1
  • fibronectin 1
  • FN1
  • fn1
  • Fn1
Spezies
Human
24
4
4
3
2
2
2
1
1
1
Quelle
Escherichia coli (E. coli)
22
7
2
1
1
1
1
1
Protein-Typ
Recombinant
Applikation
SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Optionen
'Independent Validation' Siegel
Antigen Fibronectin
Chargennummer A19042210
Validierte Anwendung Unfolding Profile
Positivkontrolle ABIN2129270
Bewertung

Passed. ABIN2129270 showed Ti at 60.9°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.

Protokoll
  • Dilute ABIN2129270 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 5µM.
  • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
  • Run Tycho measurement.
Anmerkungen

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Validierungsbilder
Unfolding Profile validation image for Fibronectin protein (ABIN2129270) Unfolding profile of ABIN2129270. The fluorescence signal is plotted against temperat...
Reinheit > 90 % pure
Proteinname
Molekulargewicht 27 kDa
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied in liquid form in 20 mM Tris-Hcl, 0.5MNaCl, 10 % glycerin ( pH 8.0) and 200 mM Imidazole
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