Oncostatin M (OSM) (AA 26-221) (Active) Protein

Details zu Produkt Nr. ABIN2039659, Anbieter: Anmelden zum Anzeigen
Proteinname
  • OSM
  • OncoM
  • oncostatin M
  • OSM
  • Osm
Proteineigenschaft
AA 26-221
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Spezies
Human
57
9
5
1
Quelle
HEK-293T Cells
51
7
3
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRR
Produktmerkmale rhOSM tested in Stat3 activation assay using glioblastoma U87 cell line.
ED50: 2.5-3 ng/mL
Reinigung Produced and purified from Serum Free/ Animal Free media
Reinheit >99 % , (according to SDS-PAGE stained with SimplyBlue SafeStain, (Invitrogen))
Sterilität 0.2 μm filtered
Endotoxin-Niveau Less than 0.1 ng/μg (1 IEU/μg), as measured by LAL method
Hintergrund Oncostatin M is a member of the gp130 (IL-6/LIF) family of cytokines. It is naturally processed from 252 amino acid pre-pro OSM polypeptide. Propeptide processing is believed not to be important for OSM receptor binding activity, but may be important for growth-inhibitory activity. Oncostatin M variable cytokine activity depends on the target cell. Oncostatin M was initially identified as a cytokine capable of inhibiting the in vitro growth of melanoma cells and solid tumors (1). It has been subsequently shown to promote proliferation of ovarian cancer cells (2) prostate cancer 22Rv1 cells (3) and AIDS-KS cells (4), up-regulate the ER chaperone Grp78/BiP in the liver cells (5), inhibit proliferation of breast cancer cell lines (6,7) and induce the activation of signaling pathways Jak2, Stat3, and Erk1/2 (8). OSM is normally expressed in macrophages, monocytes, T cells, and dendritic cells (8-10).
Molekulargewicht 22.151 kDa
Pathways JAK-STAT Signalweg, Negative Regulation of Hormone Secretion
Applikationshinweise 22 kDa, under reducing conditions.
Kommentare

outstanding bioactivity, superior lot-to-lot reproducibilty in bioactivity assays, sertificate of analysis for every lot.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Spin before opening. For optimal recovery - reconstitute in sterile water at ~0.1 mg/mL at room temperature, after adding water, re-cap the vial and tap gently, ensure to cover all the surfaces inside the vial. Do not mix by vortexing or by extensive pipetting. Let the temperature with gentle agitation for at least 10-15 minutes before aliquoting or using.vial to sit at room
Buffer Dried from 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Handhabung Avoid repeated freeze-thaw cycles.
Not for use in humans.
Lagerung -20 °C/-80 °C
Informationen zur Lagerung To avoid loss of the protein, store the reconstituted protein in aliquots (no smaller than 10 μL) in polypropylene or siliconized tubes. Store dried and reconstituted protein at -20 °C and/or -80 °C.
Bilder des Herstellers
 image for Oncostatin M (OSM) (AA 26-221) (Active) protein (ABIN2039659) Oncostatin M (OSM) (AA 26-221) (Active) protein
Produkt verwendet in: Godoy-Tundidor, Cavarretta, Fuchs, Fiechtl, Steiner, Friedbichler, Bartsch, Hobisch, Culig: "Interleukin-6 and oncostatin M stimulation of proliferation of prostate cancer 22Rv1 cells through the signaling pathways of p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase." in: The Prostate, Vol. 64, Issue 2, pp. 209-16, 2005 (PubMed).

Yin, Chain: "Suppression of lymphokine production in anti-minor histocompatibility antigen responses." in: Cytokine, Vol. 3, Issue 1, pp. 5-11, 1991 (PubMed).

Malik, Kallestad, Gunderson, Austin, Neubauer, Ochs, Marquardt, Zarling, Shoyab, Wei: "Molecular cloning, sequence analysis, and functional expression of a novel growth regulator, oncostatin M." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 2847-53, 1989 (PubMed).

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