TNF alpha Protein (AA 84-235)

Produktnummer ABIN2018408

Produktdetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 84-235

Spezies: Maus

Quelle: Yeast (Pichia pastoris)

Produktmerkmale: ED50<0.01 ng/mL, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 108 units/mg.

Reinheit: > 95 % as analyzed by reducing SDS-PAGE.

Endotoxin-Niveau: < 1 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 1 week at 4°C or up to 2 months at -20°C.

Haltbarkeit: 6 months

Referenzen

Gao, Wan, Mateo, Callegari, Wang, Deng, Puente, Li, Chaussee, Finlay, Lenardo, Hardwidge: "Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function." in: PLoS pathogens, Vol. 5, Issue 12, pp. e1000708, (2009) (PubMed).

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF-alpha (TNF alpha Produkte)

Synonyme: DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein

Hintergrund: Sharing 79 % sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2

Molekulargewicht: 17kDa, observed by reducing SDS-PAGE.

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome