KIT Ligand Protein (KITLG)

Produktnummer ABIN2018375

Produktdetails

Target: KIT Ligand (KITLG)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: The ED50, as determined by the dose-dependant stimulation of human TF-1 cells, is < 2 ng/mL, corresponding to a specific activity of 5x10^5 IU/mg.
The sequence of the first five N-terminal amino acids has been found to be Met-Glu-Gly-Ile-Cys.

Reinheit: Greater than 95.0 % as determined by the following methods:(a) Analysis by SEC-HPLC(b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel

Endotoxin-Niveau: < 0.1 ng/μg (IEU/μg) of rHuSCF

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is recommended that the lyophilized recombinant human stem cell factor (SCF) be reconstituted in sterile 18 M omega-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions.

Buffer: The protein was lyophilized after extensive dialysis against 20 mM HAc-NaAc, pH 4.5, 200 mM NaCl buffer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized recombinant human stem cell factor (SCF) remains stable at room temperature for three weeks, but it is best stored desiccated below -18 °C. Upon reconstitution Recombinant Human Stem Cell Factor (SCF) should be stored at 4 °C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added.

Referenzen

Herraiz, Journé, Abdel-Malek, Ghanem, Jiménez-Cervantes, García-Borrón: "Signaling from the human melanocortin 1 receptor to ERK1 and ERK2 mitogen-activated protein kinases involves transactivation of cKIT." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 1, pp. 138-56, (2011) (PubMed).

Woost, Solchaga, Meyerson, Shankey, Goolsby, Jacobberger: "High-resolution kinetics of cytokine signaling in human CD34/CD117-positive cells in unfractionated bone marrow." in: Blood, Vol. 117, Issue 15, pp. e131-41, (2011) (PubMed).

Antigendetails

Target: KIT Ligand (KITLG)

Andere Bezeichnung: Stem Cell Factor (SCF) (KITLG Produkte)

Synonyme: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Hintergrund: Human Stem Cell Factor (SCF), produced in E. coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18,409 Da. Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. Stem Cell Factor (SCF) stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.
Synonyms: rHuSCF, SCF

Molekulargewicht: 18,409 Da

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung