IL6RA Protein (AA 20-358, Isoform 1) (His tag)

Produktnummer ABIN2018358

Produktdetails

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Isoform 1, AA 20-358

Spezies: Human

Quelle: Insect cells (Sf9)

Aufreinigungstag / Konjugat: Dieses IL6RA Protein ist gelabelt mit His tag.

Produktmerkmale: ED50 < 50 ng/mL, measured by the cytotoxicity assay using M1 cells in presence of 10 ng/mL of human IL-6, corresponding to a specific activity of > 2x10^4 units/mg.
AA 20-358 (isoform 1), expressed with an N-terminal Histag.

Reinheit: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhsIL-6R alpha remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Antigendetails

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Abstract: IL6RA Produkte

Synonyme: IL6R1 Protein, Il6ra Protein, CD126 Protein, IL-6R-1 Protein, IL-6RA Protein, IL6Q Protein, IL6RA Protein, IL6RQ Protein, gp80 Protein, IL-6R Protein, Il6r Protein, interleukin 6 receptor Protein, interleukin 6 receptor, alpha Protein, Il6r Protein, IL6R Protein, Il6ra Protein

Hintergrund: Interleukin-6 Receptor (IL-6R) is a single trans-membrane protein that is the receptor for Interleukin-6 (IL-6). IL-6R forms a hexameric complex upon binding 2 Molecules of IL-6 and two molecules of glycoprotein 130 (gp130) which activates intracellular JAK/STAT pathways. Although the normal form of IL-6R is the membrane-bound 80 kDa subunit, a soluble form of IL-6R (sIL-6R) can be generated physiologically by limited proteolysis or alternative splicing. sIL-6R binds to both IL-6 and gp130 generating intracellular signaling. In the immune system, sIL-6R is produced by both na ve and memory CD4 T-cells and strongly augments IL-6 ligand's induction of Th-17 cells.Recombinant human Soluble IL-6 Receptor Alpha (rhsIL-6R alpha) with an N-terminal His tag produced in Sf9 insect cells is a single glycosylated polypeptide chain containing 350 amino acids. A fully biologically active molecule, rhsIL-6R alpha has a molecular mass of 50 kDa analyzed by reducing SDS-PAGE.
Synonyms: gp80

Molekulargewicht: 50 kDa, observed by reducing SDS-PAGE.

UniProt: P08887

Pathways: JAK-STAT Signalweg, Autophagie, Growth Factor Binding, Cancer Immune Checkpoints