N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 34-756), (Lumenal Region) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2007135, Anbieter: Anmelden zum Anzeigen
Proteinname
  • BCDase
  • HNAC1
  • LCDase
  • N-CDase
  • NCDase
  • AI585898
  • N-acylsphingosine amidohydrolase 2
  • ASAH2
  • Asah2
Proteineigenschaft
AA 34-756, Lumenal Region
1
1
1
1
1
1
Spezies
Maus
4
3
Quelle
Human Cells
6
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses ASAH2 Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The secreted recombinant mouse ASAH2 consists of 739 amino acids and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal polyhistidine tag.
Predicted N-Term: His
Reinheit > 97 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid.
The specific activity is > 3,000 pmoles/min/μg.
Hintergrund Synonyms: AI585898
Molekulargewicht 82 kDa, 105-115 kDa
NCBI Accession NP_061300
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 34-756), (Lumenal Region) (Active) protein (His tag) (ABIN2007135) N-Acylsphingosine Amidohydrolase (Non-Lysosomal Ceramidase) 2 (ASAH2) (AA 34-756), (Lumenal Region) (Active) protein (His tag)
Allgemeine Veröffentlichungen Kono, Dreier, Ellis, Allende, Kalkofen, Sanders, Bielawski, Bielawska, Hannun, Proia: "Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7324-31, 2006

Kono, Dreier, Ellis, Allende, Kalkofen, Sanders, Bielawski, Bielawska, Hannun, Proia: "Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids." in: The Journal of biological chemistry, Vol. 281, Issue 11, pp. 7324-31, 2006 (PubMed).

Mitsutake, Tani, Okino, Mori, Ichinose, Omori, Iida, Nakamura, Ito: "Purification, characterization, molecular cloning, and subcellular distribution of neutral ceramidase of rat kidney." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26249-59, 2001 (PubMed).

Tani, Okino, Mori, Tanigawa, Izu, Ito: "Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases." in: The Journal of biological chemistry, Vol. 275, Issue 15, pp. 11229-34, 2000 (PubMed).

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