Biotinidase (BTD) (AA 1-543) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2006595, Anbieter: Anmelden zum Anzeigen
Proteinname
  • BTD
  • CG3599
  • CT12113
  • Dmel\\CG3599
  • zgc:153333
  • Biotinase
  • Biotinidase
  • biotinidase
  • Btnd
  • btd
  • BTD
  • Btd
Proteineigenschaft
AA 1-543
1
1
1
1
1
1
1
1
1
Spezies
Human
5
1
1
1
1
Quelle
Human Cells
3
2
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses BTD Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human BTD comprises 513 amino acids and has a predicted molecular mass of 58.2 kDa. The apparent molecular mass of the protein is approximately 66-76 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Ala 42
Reinheit > 95 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/μg.
Proteinname Biotinidase (BTD)
Hintergrund Synonyms: BTD
Molekulargewicht 58.2 kDa, 66-76 kDa
UniProt P43251
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Biotinidase (BTD) (AA 1-543) (Active) protein (His tag) (ABIN2006595) Biotinidase (BTD) (AA 1-543) (Active) protein (His tag)
Allgemeine Veröffentlichungen McMahon: "Biotin in metabolism and molecular biology." in: Annual review of nutrition, Vol. 22, pp. 221-39, 2002

McMahon: "Biotin in metabolism and molecular biology." in: Annual review of nutrition, Vol. 22, pp. 221-39, 2002 (PubMed).

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