Cadherin-16 (CDH16) (AA 1-786), (Extracellular Domain) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2003602, Anbieter: Anmelden zum Anzeigen
Proteinname
  • cadherin 16
  • CDH16
  • Cdh16
Proteineigenschaft
Extracellular Domain, AA 1-786
2
1
1
1
1
1
1
1
1
Spezies
Human
8
4
Quelle
Human Cells
4
4
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human CDH16 comprises 779 amino acids and has a calculated molecular mass of 84.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100 kDa protein in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the extracellular domain of human CDH16 (NP_004053.1) (Met 1-Ala 786) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Lys 19
Reinheit > 70 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells.
When cells are added to CDH16-His coated plates (10 μg/mL, 100 μL/well), approximately 20-40% will adhere specifically after 60 minutes at 37 °C.
Hintergrund Synonyms: UNQ695/PRO1340
Molekulargewicht 84.8 kDa, 100 kDa
NCBI Accession NP_004053
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Cadherin-16 (CDH16) (AA 1-786), (Extracellular Domain) (Active) protein (His tag) (ABIN2003602) Cadherin-16 (CDH16) (AA 1-786), (Extracellular Domain) (Active) protein (His tag)
Allgemeine Veröffentlichungen Hulpiau, van Roy: "Molecular evolution of the cadherin superfamily." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 2, pp. 349-69, 2008 (PubMed).

Bayas, Leung, Evans, Leckband: "Lifetime measurements reveal kinetic differences between homophilic cadherin bonds." in: Biophysical journal, Vol. 90, Issue 4, pp. 1385-95, 2006 (PubMed).

Crosby, Fleming, Argraves, Corada, Zanetta, Dejana, Drake: "VE-cadherin is not required for the formation of nascent blood vessels but acts to prevent their disassembly." in: Blood, Vol. 105, Issue 7, pp. 2771-6, 2005 (PubMed).

Duguay, Foty, Steinberg: "Cadherin-mediated cell adhesion and tissue segregation: qualitative and quantitative determinants." in: Developmental biology, Vol. 253, Issue 2, pp. 309-23, 2003 (PubMed).

Angst, Marcozzi, Magee: "The cadherin superfamily: diversity in form and function." in: Journal of cell science, Vol. 114, Issue Pt 4, pp. 629-41, 2001 (PubMed).