Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) (AA 56-960), (Lumenal Region) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2003323, Anbieter: Anmelden zum Anzeigen
Proteinname
  • L-RAP
  • LRAP
  • ERAP2
  • zgc:172163
  • endoplasmic reticulum aminopeptidase 2
  • ERAP2
  • erap2
Proteineigenschaft
AA 56-960, Lumenal Region
1
1
1
1
1
Spezies
Human
4
Quelle
Human Cells
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human ERAP2 consists of 921 amino acids and predictes a molecular mass of 106 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 115-125 kDa due to glycosylation.

Protein Structure: A DNA sequence encoding the lumenal domain of human ERAP2 (NP_071745.1) (Ala 56-Thr 960) was expressed with a polyhistidine tag at the N-terminus.
Predicted N-Term: Leu 24
Reinheit > 98 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is >50 pmoles/min/μg.
Proteinname Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)
Hintergrund Synonyms: FLJ23633,FLJ23701,FLJ23807,L-RAP,LRAP,LRAP.ERAP2
Molekulargewicht 106 kDa, 115-125 kDa
NCBI Accession NP_071745
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, pH 7.5, 50 % glycercol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) (AA 56-960), (Lumenal Region) (Active) protein (His tag) (ABIN2003323) Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) (AA 56-960), (Lumenal Region) (Active) protein (His tag)
Allgemeine Veröffentlichungen Saveanu, Carroll, Lindo, Del Val, Lopez, Lepelletier, Greer, Schomburg, Fruci, Niedermann, van Endert: "Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum." in: Nature immunology, Vol. 6, Issue 7, pp. 689-97, 2005

Saveanu, Carroll, Lindo, Del Val, Lopez, Lepelletier, Greer, Schomburg, Fruci, Niedermann, van Endert: "Concerted peptide trimming by human ERAP1 and ERAP2 aminopeptidase complexes in the endoplasmic reticulum." in: Nature immunology, Vol. 6, Issue 7, pp. 689-97, 2005 (PubMed).

Tanioka, Hattori, Mizutani, Tsujimoto: "Regulation of the human leukocyte-derived arginine aminopeptidase/endoplasmic reticulum-aminopeptidase 2 gene by interferon-gamma." in: The FEBS journal, Vol. 272, Issue 4, pp. 916-28, 2005 (PubMed).

Tanioka, Hattori, Masuda, Nomura, Nakayama, Mizutani, Tsujimoto: "Human leukocyte-derived arginine aminopeptidase. The third member of the oxytocinase subfamily of aminopeptidases." in: The Journal of biological chemistry, Vol. 278, Issue 34, pp. 32275-83, 2003 (PubMed).

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