CA12 (AA 1-291), (Extracellular Domain) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2003146, Anbieter: Anmelden zum Anzeigen
Proteinname
  • CAXII
  • HsT18816
  • CAR12
  • 2310047E01Rik
  • AI314958
  • Ca12
  • carbonic anhydrase 12
  • CA12
  • Car12
Proteineigenschaft
Extracellular Domain, AA 1-291
6
2
1
1
1
1
1
1
1
1
1
1
Spezies
Human
9
7
2
1
Quelle
Human Cells
8
3
2
1
1
1
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Ala 25
Reinheit > 97 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Proteinname CA12
Hintergrund Synonyms: CA12,CAXII,FLJ20151,HsT18816
Molekulargewicht 31.6 kDa, 40-45 kDa
NCBI Accession NP_001209
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for CA12 (AA 1-291), (Extracellular Domain) (Active) protein (His tag) (ABIN2003146) CA12 (AA 1-291), (Extracellular Domain) (Active) protein (His tag)
Produkt verwendet in: Lecoeur, Goossens, Vaccher, Bonte, Foulon: "A multivariate approach for the determination of isoelectric point of human carbonic anhydrase isoforms by capillary isoelectric focusing." in: Electrophoresis, Vol. 32, Issue 20, pp. 2857-66, 2011 (PubMed).

Allgemeine Veröffentlichungen Ivanov, Kuzmin, Wei, Pack, Geil, Johnson, Stanbridge, Lerman: "Down-regulation of transmembrane carbonic anhydrases in renal cell carcinoma cell lines by wild-type von Hippel-Lindau transgenes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12596-601, 1998 (PubMed).

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