Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2003062, Anbieter: Anmelden zum Anzeigen
Proteinname
  • A630013F22Rik
  • AW552122
  • EPI
  • LACI
  • TFI
  • TFPI1
  • tissue factor pathway inhibitor
  • Tfpi
  • TFPI
Proteineigenschaft
AA 1-282
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
Spezies
Human
20
5
3
3
2
Quelle
Human Cells
16
8
3
2
2
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses TFPI Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The secreted recombinant human TFPI comprises 265 amino acids with a predicted molecular mass of 30.6 kDa. As a result of glycosylation, the apparent molecular mass of rhTFPI is approximately 42-45 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human TFPI (NP_006278.1) (Met 1-Lys 282) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Asp 29
Reinheit > 85 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 0.4 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij-35, pH 7.5 .
Hintergrund Synonyms: EPI,LACI,TFI,TFPI1
Molekulargewicht 30.6 kDa, 42-45 kDa
NCBI Accession NP_006278
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag) (ABIN2003062) Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-282) (Active) protein (His tag)
Produkt verwendet in: Marlu, Polack: "Gla-domainless factor Xa: molecular bait to bypass a blocked tenase complex." in: Haematologica, Vol. 97, Issue 8, pp. 1165-72, 2012 (PubMed).

Allgemeine Veröffentlichungen Bajaj, Birktoft, Steer, Bajaj: "Structure and biology of tissue factor pathway inhibitor." in: Thrombosis and haemostasis, Vol. 86, Issue 4, pp. 959-72, 2001

Bajaj, Birktoft, Steer, Bajaj: "Structure and biology of tissue factor pathway inhibitor." in: Thrombosis and haemostasis, Vol. 86, Issue 4, pp. 959-72, 2001 (PubMed).

Li, Wun: "Proteolysis of tissue factor pathway inhibitor (TFPI) by plasmin: effect on TFPI activity." in: Thrombosis and haemostasis, Vol. 80, Issue 3, pp. 423-7, 1998 (PubMed).

Ruci?ska, Gacko, Skrzydlewski: "Tissue factor pathway inhibitor (TFPI)." in: Roczniki Akademii Medycznej w Bia?ymstoku (1995), Vol. 42 Suppl 1, pp. 118-22, 1997 (PubMed).

Broze, Warren, Novotny, Higuchi, Girard, Miletich: "The lipoprotein-associated coagulation inhibitor that inhibits the factor VII-tissue factor complex also inhibits factor Xa: insight into its possible mechanism of action." in: Blood, Vol. 71, Issue 2, pp. 335-43, 1988 (PubMed).

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