Iduronate 2-Sulfatase (IDS) (AA 1-550) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2002610, Anbieter: Anmelden zum Anzeigen
Proteinname
  • mps2
  • sids
  • zgc:158245
  • MPS2
  • SIDS
  • AW214631
  • iduronate 2-sulfatase
  • IDS
  • CpipJ_CPIJ004938
  • ids
  • Ids
Proteineigenschaft
AA 1-550
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
7
3
1
Quelle
Human Cells
5
3
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses IDS Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Produktmerkmale The secreted recombinant human IDS with the propeptide consists of 536 amino acids after removal of the signal peptide and has a predicted molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, it migrates as several bands with apparent molecular mass between 85-95 KDa due to glycosylation.

Protein Structure: A DNA sequence encoding human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Ser 26
Reinheit > 87 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is > 1.0 pmoles/min/μg.
Hintergrund Synonyms: IDS,MPS2,SIDS
Molekulargewicht 61 kDa
NCBI Accession NP_000193
Pathways Glycosaminoglycan Metabolic Process
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Iduronate 2-Sulfatase (IDS) (AA 1-550) (Active) protein (His tag) (ABIN2002610) Iduronate 2-Sulfatase (IDS) (AA 1-550) (Active) protein (His tag)
Allgemeine Veröffentlichungen Sukegawa-Hayasaka, Kato, Nakamura, Tomatsu, Fukao, Kuwata, Orii, Kondo: "Effect of Hunter disease (mucopolysaccharidosis type II) mutations on molecular phenotypes of iduronate-2-sulfatase: enzymatic activity, protein processing and structural analysis." in: Journal of inherited metabolic disease, Vol. 29, Issue 6, pp. 755-61, 2006

Sukegawa-Hayasaka, Kato, Nakamura, Tomatsu, Fukao, Kuwata, Orii, Kondo: "Effect of Hunter disease (mucopolysaccharidosis type II) mutations on molecular phenotypes of iduronate-2-sulfatase: enzymatic activity, protein processing and structural analysis." in: Journal of inherited metabolic disease, Vol. 29, Issue 6, pp. 755-61, 2006 (PubMed).

Braun, Aronovich, Anderson, Crotty, McIvor, Whitley: "Metabolic correction and cross-correction of mucopolysaccharidosis type II (Hunter syndrome) by retroviral-mediated gene transfer and expression of human iduronate-2-sulfatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 24, pp. 11830-4, 1994 (PubMed).

Wilson, Morris, Anson, Occhiodoro, Bielicki, Clements, Hopwood: "Hunter syndrome: isolation of an iduronate-2-sulfatase cDNA clone and analysis of patient DNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 21, pp. 8531-5, 1990 (PubMed).

Haben Sie etwas anderes gesucht?