serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 1-418) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2002549, Anbieter: Anmelden zum Anzeigen
Proteinname
  • A1AT
  • PI
  • alpha-1-PI
  • 9530075H20Rik
  • A1A
  • AAT
  • PI1
  • PRO2275
  • alpha1AT
  • Pi
  • Spi1
  • MGC64429
  • serpina1d
  • SERPINA1
  • alpha-1-antiproteinase F precursor
  • serpin family A member 1
  • calumenin
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
  • serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog
  • LOC100008973
  • SERPINA1
  • Calu
  • CALU
  • Serpina1
  • serpina1.S
  • SPIA1
  • serpina1
  • SPIA5
  • serpina1.L
Proteineigenschaft
AA 1-418
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
30
4
2
1
1
1
1
1
1
1
1
1
Quelle
Human Cells
13
11
10
3
2
2
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses SERPINA1 Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The secreted mature form of recombinant human SerpinA1 consists of 405 amino acids and has a calculated molecular mass of 45.7 kDa. Due to glycosylation, the rhSerpinA1 migrates as an approximately 55-60 kDa protein in SDS-PAGE analysis under reducing conditions.

Protein Structure: A DNA sequence encoding the human SerpinA1 (NP_000286.3) pre-protein (Met 1-Lys 418) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Glu 25
Reinheit > 97 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog#27114) . The IC50 value is < 3.0 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog#T1426), 10 μM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.
Hintergrund Synonyms: A1A,A1AT,AAT,alpha1AT,MGC23330,MGC9222,PI,PI1,PRO2275,SerpinA1
Molekulargewicht 45.7 kDa, 55-60 kDa
NCBI Accession NP_000286
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 1-418) (Active) protein (His tag) (ABIN2002549) serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1) (AA 1-418) (Active) protein (His tag)
Allgemeine Veröffentlichungen Lomas: "The selective advantage of alpha1-antitrypsin deficiency." in: American journal of respiratory and critical care medicine, Vol. 173, Issue 10, pp. 1072-7, 2006

Lomas: "The selective advantage of alpha1-antitrypsin deficiency." in: American journal of respiratory and critical care medicine, Vol. 173, Issue 10, pp. 1072-7, 2006 (PubMed).

Burrows, Willis, Perlmutter et al.: "Chemical chaperones mediate increased secretion of mutant alpha 1-antitrypsin (alpha 1-AT) Z: A potential pharmacological strategy for prevention of liver injury and emphysema in alpha 1-AT ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 4, pp. 1796-801, 2000 (PubMed).

Perlmutter, Daniels, Auerbach, De Schryver-Kecskemeti, Winter, Alpers: "The alpha 1-antitrypsin gene is expressed in a human intestinal epithelial cell line." in: The Journal of biological chemistry, Vol. 264, Issue 16, pp. 9485-90, 1989 (PubMed).

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