Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 2-321) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2002547, Anbieter: Anmelden zum Anzeigen
Proteinname
  • PTPN1
  • PTP1B
  • PTP-1B
  • PTP-HA2
  • Ptp
  • ptp1b
  • wu:fk54h03
  • CPTP1
  • protein tyrosine phosphatase, non-receptor type 1
  • PTPN1
  • Ptpn1
  • ptpn1
Proteineigenschaft
AA 2-321
3
2
2
2
2
1
1
Spezies
Human
18
1
1
1
Quelle
Escherichia coli (E. coli)
8
2
2
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses PTPN1 Protein ist gelabelt mit His tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Produktmerkmale The recombinant human PTPN1consisting of 327 amino acids and has a calculated molecular mass of 38 kDa.

Protein Structure: A DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2-Asn 321) was expressed, with a polyhistide tag at the N-terminus.
Predicted N-Term: Met
Reinheit > 95 % as determined by SDS-PAGE
ProductDetails: Biological Activity Comment Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate, R&D Systems, Catalog # ES006.
The specific activity is > 15 nmoles/min/μg.
Hintergrund Synonyms: PTP1B,PTPN1
Molekulargewicht 38 kDa
NCBI Accession NP_002818
Pathways TLR Signalweg, Response to Growth Hormone Stimulus, ER-Nucleus Signaling, Platelet-derived growth Factor Receptor Signaling
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied as sterile 10 mM Hepes, 150 mM NaCl, 1 mM DTT, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Liquid. It is shipped out with blue ice.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 2-321) (Active) protein (His tag) (ABIN2002547) Protein tyrosine Phosphatase, Non-Receptor Type 1 (PTPN1) (AA 2-321) (Active) protein (His tag)
Allgemeine Veröffentlichungen Liang, Lee, Liang, Lawrence, Zhang: "The role of protein-tyrosine phosphatase 1B in integrin signaling." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24857-63, 2005

Liang, Lee, Liang, Lawrence, Zhang: "The role of protein-tyrosine phosphatase 1B in integrin signaling." in: The Journal of biological chemistry, Vol. 280, Issue 26, pp. 24857-63, 2005 (PubMed).

Haj, Verveer, Squire, Neel, Bastiaens: "Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum." in: Science (New York, N.Y.), Vol. 295, Issue 5560, pp. 1708-11, 2002 (PubMed).

Moeslein, Myers, Landreth: "The CLK family kinases, CLK1 and CLK2, phosphorylate and activate the tyrosine phosphatase, PTP-1B." in: The Journal of biological chemistry, Vol. 274, Issue 38, pp. 26697-704, 1999 (PubMed).

Frangioni, Beahm, Shifrin, Jost, Neel: "The nontransmembrane tyrosine phosphatase PTP-1B localizes to the endoplasmic reticulum via its 35 amino acid C-terminal sequence." in: Cell, Vol. 68, Issue 3, pp. 545-60, 1992 (PubMed).

Haben Sie etwas anderes gesucht?