Complement C2 (AA 1-752) (Active) protein (His tag)

Details zu Produkt Nr. ABIN2002185, Anbieter: Anmelden zum Anzeigen
Proteinname
  • CO2
  • complement C2
  • complement component 2 (within H-2S)
  • C2
Proteineigenschaft
AA 1-752
2
2
2
1
1
1
1
1
1
1
1
1
Spezies
Human
9
4
1
Quelle
Human Cells
6
3
3
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The single-chain form of recombinant human complement component C2 comprises 743 amino acids and has a calculated molecular mass of 82.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhC2 is 90-100 kDa as a result of glycosylation.

Protein Structure: A DNA sequence encoding the human complement component 2 (C2) precursor (NP_000054.2) (Met 1-Leu 752) was expressed with a C-terminal polyhistidine tag.
Predicted N-Term: Ala 21
Reinheit > 97 % as determined by SDS-PAGE and SEC-HPLC Analysis.
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5'Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmoles/min/μg.
Hintergrund Synonyms: ARMD14,CO2
Molekulargewicht 82.5 kDa, 90-100 kDa
NCBI Accession NP_000054
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Complement C2 (AA 1-752) (Active) protein (His tag) (ABIN2002185) Complement C2 (AA 1-752) (Active) protein (His tag)
Allgemeine Veröffentlichungen Wallis, Dodds, Mitchell, Sim, Reid, Schwaeble: "Molecular interactions between MASP-2, C4, and C2 and their activation fragments leading to complement activation via the lectin pathway." in: The Journal of biological chemistry, Vol. 282, Issue 11, pp. 7844-51, 2007 (PubMed).

Alper, Xu, Cosmopoulos, Dolinski, Stein, Uko, Larsen, Dubey, Densen, Truedsson, Sturfelt, Sjöholm: "Immunoglobulin deficiencies and susceptibility to infection among homozygotes and heterozygotes for C2 deficiency." in: Journal of clinical immunology, Vol. 23, Issue 4, pp. 297-305, 2003 (PubMed).

Wang, Circolo, Lokki, Shackelford, Wetsel, Colten: "Molecular heterogeneity in deficiency of complement protein C2 type I." in: Immunology, Vol. 93, Issue 2, pp. 184-91, 1998 (PubMed).

Akama, Johnson, Colten: "Human complement protein C2. Alternative splicing generates templates for secreted and intracellular C2 proteins." in: The Journal of biological chemistry, Vol. 270, Issue 6, pp. 2674-8, 1995 (PubMed).

Bentley, Porter: "Isolation of cDNA clones for human complement component C2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 4, pp. 1212-5, 1984 (PubMed).

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