Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag)

Details zu Produkt Nr. ABIN2001973, Anbieter: Anmelden zum Anzeigen
Proteinname
  • CLMF
  • CLMF2
  • IL-12B
  • NKSF
  • NKSF2
  • p40
  • Il-12b
  • Il12p40
  • Il-12p40
  • Il12
  • IL-12p40
  • IL-12
  • IL-12 p40
  • il12b
  • il12p40.b
  • zgc:152789
  • IL-12.p40
  • IL12p40
  • IL12B
  • CLMF p40
  • interleukin 12B
  • interleukin 12b
  • interleukin 12Ba
  • IL12B
  • Il12b
  • il12ba
Proteineigenschaft
AA 1-328, Subunit p40
13
9
5
5
5
4
2
2
2
2
1
1
1
1
1
1
1
1
Spezies
Human
34
15
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
Human Cells
26
15
12
7
6
5
3
2
1
1
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses IL12B Protein ist gelabelt mit Fc Tag.
Applikation
Functional Studies (Func), SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant human IL12B consists of 544 amino acids and has a predicted molecular mass of 61.4 kDa.

Protein Structure: A DNA sequence encoding the p40 subunit of human IL12, termed asIL12B (NP_002178.2) (Met 1-Ser 328) was fused with the Fc region ofhuman IgG1 at the C-terminus.
Predicted N-Term: Ile 23
Reinheit > 85 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its binding ability in a functional ELISA . Immobilized human IL12RB1 at 20 μg/ml (100 μl/well) can bind human IL12B with a linear ranger of 2.56-64 ng/ml.
Proteinname
Hintergrund Synonyms: CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2
Molekulargewicht 61.4 kDa
NCBI Accession NP_002178
Pathways JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag) (ABIN2001973) Interleukin 12b (IL12B) (AA 1-328), (Subunit p40) (Active) protein (Fc Tag)
Allgemeine Veröffentlichungen Kim, Loveland, Sitz, Ratto Kim, Mclinden, Tencer, Davis, Burke, Boswell, Redfield, Birx: "Expansion of restricted cellular immune responses to HIV-1 envelope by vaccination: IL-7 and IL-12 differentially augment cellular proliferative responses to HIV-1." in: Clinical and experimental immunology, Vol. 108, Issue 2, pp. 243-50, 1997 (PubMed).

Thierfelder, van Deursen, Yamamoto, Tripp, Sarawar, Carson, Sangster, Vignali, Doherty, Grosveld, Ihle: "Requirement for Stat4 in interleukin-12-mediated responses of natural killer and T cells." in: Nature, Vol. 382, Issue 6587, pp. 171-4, 1996 (PubMed).

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, 1995

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, 1995 (PubMed).

Sieburth, Jabs, Warrington, Li, Lasota, LaForgia, Kelleher, Huebner, Wasmuth, Wolf: "Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5." in: Genomics, Vol. 14, Issue 1, pp. 59-62, 1992 (PubMed).

Gearing, Cosman: "Homology of the p40 subunit of natural killer cell stimulatory factor (NKSF) with the extracellular domain of the interleukin-6 receptor." in: Cell, Vol. 66, Issue 1, pp. 9-10, 1991 (PubMed).