IgG1 (AA 98-324), (Cys102Ser-Mutant) Protein

Details zu Produkt Nr. ABIN2001821, Anbieter: Anmelden zum Anzeigen
Proteinname
  • IgG1
  • Igh-4
  • VH7183
  • immunoglobulin heavy constant gamma 1 (G1m marker)
  • Ighg1
Proteineigenschaft
AA 98-324, Cys102Ser-Mutant
Spezies
Maus
5
4
1
1
Quelle
Human Cells
4
4
2
1
Protein-Typ
Recombinant
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The recombinant mouse IgG1 Fc consists of 227 amino acids and has a predicted molecular mass of 25.8 kDa. As a result of glycosylation, the apparent molecular mass of rmFc is approximately 32 kDa in SDS-PAGE under reducing conditions.

Protein Structure: The Fc region of mouse IgG1 (P01868-1) (Val 98-Lys 324) (one aa mutation, 102 Cys / Ser) was expressed and purified.
Predicted N-Term: Val 98
Reinheit > 97 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Proteinname
Hintergrund Synonyms: IgG1,Igh-4,VH7183
Molekulargewicht 25.8 kDa, 32 kDa
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile 100 mM NaAc, 10 mM NaCl, 200 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for IgG1 (AA 98-324), (Cys102Ser-Mutant) protein (ABIN2001821) IgG1 (AA 98-324), (Cys102Ser-Mutant) protein
Produkt verwendet in: Lee, Lee, Kwon, Lee, Cho, Kim, Lee, Kim, Lee, Yang, Mook-Jung, Nam, Chung, Lazar, Kim: "Adenylyl cyclase-associated protein 1 is a receptor for human resistin and mediates inflammatory actions of human monocytes." in: Cell metabolism, Vol. 19, Issue 3, pp. 484-97, 2014 (PubMed).

Allgemeine Veröffentlichungen Aybay: "Differential binding characteristics of protein G and protein A for Fc fragments of papain-digested mouse IgG." in: Immunology letters, Vol. 85, Issue 3, pp. 231-5, 2003

Aybay: "Differential binding characteristics of protein G and protein A for Fc fragments of papain-digested mouse IgG." in: Immunology letters, Vol. 85, Issue 3, pp. 231-5, 2003 (PubMed).

Wilson, Wu, Peluso, Nock: "Improved method for pepsinolysis of mouse IgG(1) molecules to F(ab')(2) fragments." in: Journal of immunological methods, Vol. 260, Issue 1-2, pp. 29-36, 2002 (PubMed).

Wernersson, Karlsson, Dahlström, Mattsson, Verbeek, Heyman: "IgG-mediated enhancement of antibody responses is low in Fc receptor gamma chain-deficient mice and increased in Fc gamma RII-deficient mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 2, pp. 618-22, 1999 (PubMed).

Tartour, de la Salle, de la Salle, Teillaud, Camoin, Galinha, Latour, Hanau, Fridman, Sautès: "Identification, in mouse macrophages and in serum, of a soluble receptor for the Fc portion of IgG (Fc gamma R) encoded by an alternatively spliced transcript of the Fc gamma RII gene." in: International immunology, Vol. 5, Issue 8, pp. 859-68, 1993 (PubMed).

Berneman, Ternynck, Avrameas: "Natural mouse IgG reacts with self antigens including molecules involved in the immune response." in: European journal of immunology, Vol. 22, Issue 3, pp. 625-33, 1992 (PubMed).

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