Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag)

Details zu Produkt Nr. ABIN1981684, Anbieter: Anmelden zum Anzeigen
Proteinname
  • KAT4
  • KATIV
  • mitAAT
  • AST
  • ISSD
  • NSD
  • SD
  • SIALIN
  • SIASD
  • SLD
  • AL022787
  • FABP-pm
  • Got-2
  • mAspAT
  • ASPATA
  • got2
  • zgc:66329
  • glutamic-oxaloacetic transaminase 2
  • solute carrier family 17 member 5
  • glutamatic-oxaloacetic transaminase 2, mitochondrial
  • glutamic-oxaloacetic transaminase 2a, mitochondrial
  • glutamic-oxaloacetic transaminase 2 L homeolog
  • GOT2
  • SLC17A5
  • got2
  • Got2
  • got2a
  • got2.L
Proteineigenschaft
AA 1-413
9
2
1
1
1
1
1
1
1
Spezies
Human
12
4
1
1
1
1
1
1
1
Quelle
Escherichia coli (E. coli)
12
7
2
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
His tag
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz MGSSHHHHHH SSGLVPRGSH MGSSSWWTHV EMGPPDPILG VTEAFKRDTN SKKMNLGVGA YRDDNGKPYV LPSVRKAEAQ IAAKNLDKEY LPIGGLAEFC KASAELALGE NSEVLKSGRF VTVQTISGTG ALRIGASFLQ RFFKFSRDVF LPKPTWGNHT PIFRDAGMQL QGYRYYDPKT CGFDFTGAVE DISKIPEQSV LLLHACAHNP TGVDPRPEQW KEIATVVKKR NLFAFFDMAY QGFASGDGDK DAWAVRHFIE QGINVCLCQS YAKNMGLYGE RVGAFTMVCK DADEAKRVES QLKILIRPMY SNPPLNGARI AAAILNTPDL RKQWLQEVKV MADRIIGMRT QLVSNLKKEG STHNWQHITD QIGMFCFTGL KPEQVERLIK EFSIYMTKDG RISVAGVTSS NVGYLAHAIH QVTK
Produktmerkmale Molecule Information: 48.4 kDa (433 aa, 1-413 aa + His Tag)
Reinheit ≥95 % by SDS-PAGE
Biological Activity Comment Specific activity is > 44 unit/mg, in which one unit will convert 1.0 μmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Unit Definition One unit will convert 1.0 μmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.
Hintergrund GOT2, also known as glutamate oxaloacetate transaminase 2, belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Glutamate oxaloacetate transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternative Names: Aspartate aminotransferase 2, mitochondrial, KAT4, KATIV, mitAAT
Molekulargewicht 48.4 kDa
Gen-ID 14719
UniProt P05202
Pathways Monocarboxylic Acid Catabolic Process
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 0.5 mg/mL solution 20 mM Tris-HCl buffer ( pH 8.0), 10 % glycerol, 150 mM NaCl and 1 mM DTT.
Konservierungs-mittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Centrifuge the vial prior to opening.
Lagerung -20 °C
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag) (ABIN1981684) Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2) (AA 1-413) (Active) protein (His tag)
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