MANF Protein (AA 25-182) (His tag)

Produktnummer ABIN1945411

Produktdetails

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Protein-Typ: Recombinant

Proteineigenschaft: AA 25-182

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses MANF Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human IL-1 Receptor-Like 2/IL-1RL2 (C-6His)

Sequenz: DGCKDIFMKN EILSASQPFA FNCTFPPITS GEVSVTWYKN SSKIPVSKII QSRIHQDETW ILFLPMEWGD SGVYQCVIKG RDSCHRIHVN LTVFEKHWCD TSIGGLPNLS DEYKQILHLG KDDSLTCHLH FPKSCVLGPI KWYKDCNEIK GERFTVLETR LLVSNVSAED RGNYACQAIL THSGKQYEVL NGITVSITER AGYGGSVPKI IYPKNHSIEV QLGTTLIVDC NVTDTKDNTN LRCWRVNNTL VDDYYDESKR IREGVETHVS FREHNLYTVN ITFLEVKMED YGLPFMCHAG VSTAYIILQL PAPDFRAYHH HHHH

Produktmerkmale: Recombinant Human Mesencephalic astrocyte-derived neurotrophic factor/MANF is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (AA 25-182) of Human MANF fused with a polyhistidine tag at the C-termin

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Andere Bezeichnung: Mesencephalic Astrocyte-Derived Neurotrophic Factor/MANF (MANF Produkte)

Synonyme: ARMET Protein, ARP Protein, 3230402M22Rik Protein, AA407711 Protein, AA408789 Protein, AA673178 Protein, Armet Protein, D17914 Protein, D18Mgi17 Protein, MANF Protein, RBM15B Protein, ARP-like Protein, CG7013 Protein, DmMANF Protein, Dmel\\CG7013 Protein, anon-EST:ParkEST188 Protein, arp Protein, armet Protein, im:6909481 Protein, zgc:153327 Protein, manf Protein, mesencephalic astrocyte derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor Protein, RNA binding motif protein 15B Protein, Mesencephalic astrocyte-derived neurotrophic factor Protein, mesencephalic astrocyte-derived neurotrophic factor S homeolog Protein, MANF Protein, Manf Protein, RBM15B Protein, manf Protein, manf.S Protein

Hintergrund: MANF also called ARMET or ARP, is short for Mesencephalic astrocyte- derived neurotrophic factor. It is a 182 aa. protein with a 24 aa. signal peptide. The N-terminal region of MANF may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response. It is secreted and can be inducted by endoplasmic reticulum stress. This protein selectively promotes the survival of dopaminergic neurons of the ventral mid-brain, and can modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. It also enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons and inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.

Molekulargewicht: 37 kDa

UniProt: Q9HB29